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Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions, then 1 g of total RNA was prepared for cDNA library construction using a cDNA-PCR Sequencing Kit (SQK-PCS109, Oxford Nanopore Technologies Ltd., Oxford, UK)

J Pharmacokinet Biopharm 7(1):2946 Choi MS, Yu JS, Yoo HH, Kim DH (2018) The role of gut microbiota in the pharmacokinetics of antihypertensive drugs

As such, the mechanism underpinning the induction of eryptosis by heavy metals is of considerable interest, and it is under investigation in various studies

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Although the responses on the SCAR-Q were strongly supportive of the benefits of the CO 2 laser, the remaining subjects could potentially alter this conclusion